However, more evaluations in the coming years are necessary for better comparisons and conclusions regarding experimental and human BZ treatment efficacy. were BZ resistant. However, beneficial effect Nocodazole was exhibited because significant reduction (p 0.05%) and/or suppression of parasitaemia was observed in mice infected with all strains (acute phase), associated to reduction/removal of inflammation and fibrosis for two/eight strains. BZ offered some benefit, even in not cured animals, what suggest that BZ use may be recommended at least for recent chronic contamination of the analyzed region. is an important tropical disease that affects 10 million people worldwide. Most infections occur in Latin America, where this disease is usually endemic. It is estimated that over 10,000 people pass away per year due to the clinical manifestations of Chagas disease, which mainly affects the heart and the gastrointestinal tract (WHO 2010 ). Benznidazole (BZ) is the only drug available for the specific treatment of human Chagas disease in Brazil (MS/SVS 2005). Differences in drug susceptibility of strains obtained from different geographic areas have been experimentally decided (Schlemper Jr 1982, Andrade et al. 1985 , 1989, 1992, Filardi & Brener 1987, Toledo et al. 1997 , 2002, 2003, Teston et al. 2013). Nocodazole The presence of strains that are naturally resistant to BZ and nifurtimox (NFX) (Filardi & Brener 1987, Andrade et al. 1992, Toledo et al. 1997) is an important fact that explains the low remedy rates observed in the majority of treated patients. It is well established that is a complex taxon that exhibits great genetic diversity. is usually distributed in six (I-VI) discrete taxonomic models (DTUs) (Zingales et al. 2009 ) that show significant differences related to their ecological and geographic distributions (Zingales et al. 2012). Moreover, several studies have experimentally demonstrated a significant link between the genetic diversity of strains and their biological properties (Andrade & Magalh?es 1977, de Lana et al. 1998 , Revollo et al. 1998, Toledo et al. 2002), including susceptibility to chemotherapeutic brokers (Andrade et al. 1985, 1989, Filardi & Brener 1987, Toledo et al. 2003) in human and experimental conditions (Andrade et al. 1992). As the presence of parasites is essential for initiating and maintaining the pathogenic process, it FZD4 is important to verify the capacity of BZ to eradicate parasites from tissues (Brener 1962, Toledo et al. 1997, 2004, Garcia et al. 2005). Studies in mice have shown that in addition to reducing parasite burden, BZ therapy can also reduce tissue damage (Andrade et al. 1989, 1991, Higuchi et al. 1993, Segura et al. 1994, Toledo et al. 1997, 2004, Garcia et al. 2005). The drug susceptibility ofT. cruzistrains isolated from patients with different clinical forms of the disease has been evaluated (Andrade et al. 1985, 1992, Filardi & Brener 1987, Toledo et al. 1997, Oliveira-Silva 2013). These studies can help clinicians determine when it is appropriate to Nocodazole treat patients with Chagas disease (Andrade et al. 1985), which was the main purpose of the Benznidazole Evaluation for Interrupting Trypanosomiasis (BENEFIT) project (Marin-Neto et al. 2009). Our team has analyzed Chagas disease in the municipalities of Berilo and Jos Gon?alves de Minas, located in the Jequitinhonha Valley, state of Minas Gerais (MG), Brazil, which is considered to be one of the most important endemic areas of this disease in our country (Dias et al. 1985). The study location can be considered representative of the central and southern regions of Brazil, where the majority of patients are infected with parasites of the strains in these municipalities and that these characteristics may range even within the same DTU or genetic group or clones originating from the same strain (Camandaroba et al. 2003), the goal of the present work was to evaluate the experimental efficiency of BZ in the severe and chronic stages of infections in mice contaminated using the strains that are predominant in this area (Oliveira-Silva 2013). The strains had been isolated from kids from the Jequitinhonha Valley before treatment with BZ and they’re still under evaluation. Topics, MATERIALS AND Strategies strains (501, 795, 806, 817, 829, 855, 1661 and 2405) isolated from kids with a recently available chronic infections by haemoculture (Hm) ahead of treatment (Filardi & Brener 1987) had been evaluated. Six strains were identified within a serological analysis of Chagas disease in Jos and Berilo Gon?alves de Minas, two municipalities that are in close closeness (Borges et al. 2006). Subsequently, two other strains isolated from kids before treatment were contained in the scholarly research. All strains had been categorized as – Feminine.